No problemo,
keep in mind, Youtube's 100mb limit, and 10 minute time restriction.
Take a look at the tutorials, bigger is not always better.
If the file is so large, it takes a long time to load for the viewer, they may just go to the next video.
Also, if you are using 16:9, or some other widescreen ratio, YT will not display it correctly. You will need to make your 16:9 video, then re-render into a 4:3, with the black lines at top and bottom.
THEN convert to FLV.
Sounds like a bit of work, but after you do it once or twice, you'll be able to do it in your sleep.
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